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sp6 promoter  (Integrated DNA Technologies)


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    Structured Review

    Integrated DNA Technologies sp6 promoter
    Sp6 Promoter, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sp6+promoter/SP6+Promoter/custom%4051-01-19-05%4042341748
    Average 94 stars, based on 31 article reviews
    sp6 promoter - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: cell lines were routinely verified for relevant knockouts. All plasmids used were cloned by Gibson assembly or site-directed mutagenesis and verified by sequencing. All DNA fragments were ordered from IDT or Twist Bioscience and not further verified. All cDNAs used for in vitro transcription were based on an SP64 plasmid backbone containing an SP6 promoter and lacking the polyA sequence. The follow

    In Vitro:

    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: cell lines were routinely verified for relevant knockouts. All plasmids used were cloned by Gibson assembly or site-directed mutagenesis and verified by sequencing. All DNA fragments were ordered from IDT or Twist Bioscience and not further verified. All cDNAs used for in vitro transcription were based on an SP64 plasmid backbone containing an SP6 promoter and lacking the polyA sequence. The follow

    Mutagenesis:

    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: cell lines were routinely verified for relevant knockouts. All plasmids used were cloned by Gibson assembly or site-directed mutagenesis and verified by sequencing. All DNA fragments were ordered from IDT or Twist Bioscience and not further verified. All cDNAs used for in vitro transcription were based on an SP64 plasmid backbone containing an SP6 promoter and lacking the polyA sequence. The follow

    Clone Assay:

    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: cell lines were routinely verified for relevant knockouts. All plasmids used were cloned by Gibson assembly or site-directed mutagenesis and verified by sequencing. All DNA fragments were ordered from IDT or Twist Bioscience and not further verified. All cDNAs used for in vitro transcription were based on an SP64 plasmid backbone containing an SP6 promoter and lacking the polyA sequence. The follow

    Plasmid Preparation:

    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: cell lines were routinely verified for relevant knockouts. All plasmids used were cloned by Gibson assembly or site-directed mutagenesis and verified by sequencing. All DNA fragments were ordered from IDT or Twist Bioscience and not further verified. All cDNAs used for in vitro transcription were based on an SP64 plasmid backbone containing an SP6 promoter and lacking the polyA sequence. The follow

    Sequencing:

    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: cell lines were routinely verified for relevant knockouts. All plasmids used were cloned by Gibson assembly or site-directed mutagenesis and verified by sequencing. All DNA fragments were ordered from IDT or Twist Bioscience and not further verified. All cDNAs used for in vitro transcription were based on an SP64 plasmid backbone containing an SP6 promoter and lacking the polyA sequence. The follow

    Concentration Assay:

    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: cell lines were routinely verified for relevant knockouts. All plasmids used were cloned by Gibson assembly or site-directed mutagenesis and verified by sequencing. All DNA fragments were ordered from IDT or Twist Bioscience and not further verified. All cDNAs used for in vitro transcription were based on an SP64 plasmid backbone containing an SP6 promoter and lacking the polyA sequence. The follow

    Polymerase Chain Reaction:

    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: cell lines were routinely verified for relevant knockouts. All plasmids used were cloned by Gibson assembly or site-directed mutagenesis and verified by sequencing. All DNA fragments were ordered from IDT or Twist Bioscience and not further verified. All cDNAs used for in vitro transcription were based on an SP64 plasmid backbone containing an SP6 promoter and lacking the polyA sequence. The follow

    Synthesized:

    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: cell lines were routinely verified for relevant knockouts. All plasmids used were cloned by Gibson assembly or site-directed mutagenesis and verified by sequencing. All DNA fragments were ordered from IDT or Twist Bioscience and not further verified. All cDNAs used for in vitro transcription were based on an SP64 plasmid backbone containing an SP6 promoter and lacking the polyA sequence. The follow

    Cloning:

    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: cell lines were routinely verified for relevant knockouts. All plasmids used were cloned by Gibson assembly or site-directed mutagenesis and verified by sequencing. All DNA fragments were ordered from IDT or Twist Bioscience and not further verified. All cDNAs used for in vitro transcription were based on an SP64 plasmid backbone containing an SP6 promoter and lacking the polyA sequence. The follow

    Amplification:

    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: cell lines were routinely verified for relevant knockouts. All plasmids used were cloned by Gibson assembly or site-directed mutagenesis and verified by sequencing. All DNA fragments were ordered from IDT or Twist Bioscience and not further verified. All cDNAs used for in vitro transcription were based on an SP64 plasmid backbone containing an SP6 promoter and lacking the polyA sequence. The follow

    Recombinant:

    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: cell lines were routinely verified for relevant knockouts. All plasmids used were cloned by Gibson assembly or site-directed mutagenesis and verified by sequencing. All DNA fragments were ordered from IDT or Twist Bioscience and not further verified. All cDNAs used for in vitro transcription were based on an SP64 plasmid backbone containing an SP6 promoter and lacking the polyA sequence. The follow

    Binding Assay:

    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: cell lines were routinely verified for relevant knockouts. All plasmids used were cloned by Gibson assembly or site-directed mutagenesis and verified by sequencing. All DNA fragments were ordered from IDT or Twist Bioscience and not further verified. All cDNAs used for in vitro transcription were based on an SP64 plasmid backbone containing an SP6 promoter and lacking the polyA sequence. The follow

    Construct:

    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: cell lines were routinely verified for relevant knockouts. All plasmids used were cloned by Gibson assembly or site-directed mutagenesis and verified by sequencing. All DNA fragments were ordered from IDT or Twist Bioscience and not further verified. All cDNAs used for in vitro transcription were based on an SP64 plasmid backbone containing an SP6 promoter and lacking the polyA sequence. The follow

    Flow Cytometry:

    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: cell lines were routinely verified for relevant knockouts. All plasmids used were cloned by Gibson assembly or site-directed mutagenesis and verified by sequencing. All DNA fragments were ordered from IDT or Twist Bioscience and not further verified. All cDNAs used for in vitro transcription were based on an SP64 plasmid backbone containing an SP6 promoter and lacking the polyA sequence. The follow

    Cytometry:

    Article Title: Pairwise transmembrane domain insertion during multipass protein biogenesis.
    Article Snippet: cell lines were routinely verified for relevant knockouts. All plasmids used were cloned by Gibson assembly or site-directed mutagenesis and verified by sequencing. All DNA fragments were ordered from IDT or Twist Bioscience and not further verified. All cDNAs used for in vitro transcription were based on an SP64 plasmid backbone containing an SP6 promoter and lacking the polyA sequence. The follow



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    A) Plasmid map of pGEM-T illustrates the position of T-overhangs used in T/A cloning and flanking T7 and <t>SP6</t> promoters. B) Percentage of cDNA insertion events in the sense orientation relative to the T7 promoter (yellow) and the SP6 promoter (blue) in constructs generated over the course of four CURE projects. The average across the projects is shown (checkered graph). C) Measured distribution of insert orientation from a cloning attempt of SMEST023171001.1 ( pumilio homolog) cDNA fragment assessed after the ligation and transformant selection steps. D) Bar graphs display orientation distribution of inserts after the ligation step from the cDNA fragment in (C; Gene 1) and two additional genes (SMEST004045004.1, Gene 2; and SMEST012773001.1, Gene 3), as well as their average (checkered bar). Orientation of Control Insert DNA ( luci ) included in the pGEM-T Vector System after the ligation step is also shown. Asterisks show statistical significance according to (* = p < 0.005) the chi-squared test and (*** = p < 0.0005) unpaired Student’s t- test.
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    Image Search Results


    A) Plasmid map of pGEM-T illustrates the position of T-overhangs used in T/A cloning and flanking T7 and SP6 promoters. B) Percentage of cDNA insertion events in the sense orientation relative to the T7 promoter (yellow) and the SP6 promoter (blue) in constructs generated over the course of four CURE projects. The average across the projects is shown (checkered graph). C) Measured distribution of insert orientation from a cloning attempt of SMEST023171001.1 ( pumilio homolog) cDNA fragment assessed after the ligation and transformant selection steps. D) Bar graphs display orientation distribution of inserts after the ligation step from the cDNA fragment in (C; Gene 1) and two additional genes (SMEST004045004.1, Gene 2; and SMEST012773001.1, Gene 3), as well as their average (checkered bar). Orientation of Control Insert DNA ( luci ) included in the pGEM-T Vector System after the ligation step is also shown. Asterisks show statistical significance according to (* = p < 0.005) the chi-squared test and (*** = p < 0.0005) unpaired Student’s t- test.

    Journal: bioRxiv

    Article Title: Directionality bias in T/A cloning

    doi: 10.64898/2026.02.11.705383

    Figure Lengend Snippet: A) Plasmid map of pGEM-T illustrates the position of T-overhangs used in T/A cloning and flanking T7 and SP6 promoters. B) Percentage of cDNA insertion events in the sense orientation relative to the T7 promoter (yellow) and the SP6 promoter (blue) in constructs generated over the course of four CURE projects. The average across the projects is shown (checkered graph). C) Measured distribution of insert orientation from a cloning attempt of SMEST023171001.1 ( pumilio homolog) cDNA fragment assessed after the ligation and transformant selection steps. D) Bar graphs display orientation distribution of inserts after the ligation step from the cDNA fragment in (C; Gene 1) and two additional genes (SMEST004045004.1, Gene 2; and SMEST012773001.1, Gene 3), as well as their average (checkered bar). Orientation of Control Insert DNA ( luci ) included in the pGEM-T Vector System after the ligation step is also shown. Asterisks show statistical significance according to (* = p < 0.005) the chi-squared test and (*** = p < 0.0005) unpaired Student’s t- test.

    Article Snippet: To tease this apart, a fragment corresponding to a planarian Pumilio homolog (SMEST023171001.1) was amplified from cDNA and ligated into pGEM-T. A portion of the ligation reaction was transformed into Escherichia coli JM109 cells, while another portion of the ligation reaction was used directly as template to sequence across the insertion site between the T7 and SP6 promoter using third generation long read sequencing (PCR-EZ, Azenta).

    Techniques: Plasmid Preparation, Cloning, Construct, Generated, Ligation, Selection, Control